Date of Award
Doctor of Philosophy (PhD)
Richard E. Lee, Ph.D.
Sarka Beranova, Ph.D. Cynthia Jeffries, B.S. Wei Li, Ph.D. Vivian Loveless, Pharm.D.
Buruli Ulcer, Mass Spectrometry, Mycolactone, Tuberculosis
The widespread availability of antimicrobial chemotherapeutics over the last half of the twentieth century has offered dramatic increases in life expectancy. Unfortunately, many pathogenic agents are exhibiting ever increasing resistance to many frontline antibiotics. New chemotherapeutic agents are urgently needed to combat this threat; this work seeks to illustrate applications in which mass spectrometric techniques may be applied to the investigation of novel, small molecule chemotherapeutics for the treatment of bacterial infections. Chapter 1 contains an introduction to mass spectrometry, as well as an overview of relevant chromatographic techniques. Chapter 2 introduces the bacterium F tularensis and M ulcerans and details the MS based investigation of their secreted virulence factors. Chapter 3 introduces a human pathogen of particular concern, M tuberculosis, and contains a detailed reporting on the MS based investigation of internally synthesized compounds for the treatment of latent Tb.
Scarborough, Jerrod Stephen , "High Resolution Mass Spectrometry (HRMS) Based Investigation of Small Molecule, Bioactive Secondary Metabolites as Probes in the Examination of Bacterial Resistance and Virulence" (2012). Theses and Dissertations (ETD). Paper 232. http://dx.doi.org/10.21007/etd.cghs.2012.0279.