Date of Award

9-2020

Document Type

Thesis

Degree Name

Master of Science (MS)

Program

Biomedical Sciences

Track

Neurosciences

Research Advisor

Detlef H. Heck, PhD

Committee

Victor V. Chizhikov, PhD Matthew Ennis, PhD

Abstract

Evidence for a cerebellar role during cardiopulmonary challenges has long been established, but investigation into cerebellar involvement in eupneic breathing has been inconclusive. Given the view of the cerebellum (CRB) as a temporally coordinating structure, any investigation into the CRB during respiration must evaluate rhythm and variability of the respiratory sequence. In this study, we chose an elegant model of cerebellar neuropathology, Atoh1-En1/2 CKO, where mutant animals have conditional loss of the developmental patterning gene Engrailed 1/2 in rhombic lip-lineage neurons and exhibit a proportional scaling-down of neuron number in hypoplastic lobules of the CRB. We utilized whole-body unrestrained plethysmography to measure respiration during eupnea, and evaluated the respiratory sequence of mutant animals and their control littermates using the average rate, the coefficient of variation (CV), and a unique measure of intrinsic rhythmicity called CV2. Linear regression analyses revealed that mutant animals had decreased overall variability and increased intrinsic rhythmicity (as measured by CV and CV2, respectively) compared to their control littermates, but we found no effect of strain on average respiratory rate. Analysis also revealed modestly decreased respiratory rates, increased CV, and increased CV2 in female animals, independent of strain. These results align well with previously reported studies and add new insight into CRB involvement in eupneic respiratory rhythmicity. Although not investigated in this study, future works should consider the coordination of breathing, licking, swallowing, and whisking in order to build a more complete understanding of the relationship between the CRB and respiration.

Declaration of Authorship

Declaration of Authorship is included in the supplemental files.

ORCID

https://orcid.org/0000-0001-5391-607X

DOI

10.21007/etd.cghs.2020.0528

2020-024-Taylor-DOA.pdf (126 kB)
Declaration of Authorship

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