Date of Award
Master of Science (MS)
Cancer and Developmental Biology
R. Kiplin Guy, PhD
Mark E. Hatley, MD, PhD Gerard P. Zambetti, PhD
cell reprogramming, cell signaling, epigenetic modification, induced pluripotent stem cell (iPSC), small molecule compound
The generation of induced pluripotent stem cells (iPSCs) through the use of small molecule compounds has evolved as a potential cellular reprogramming strategy. Individually, specific small molecule compounds have previously been shown to replace reprogramming transcription factors or enhance the efficiency of cellular reprogramming. More recently, a combination of small molecule compounds can replace all of the reprogramming factors. In this review, we describe in detail the generation of chemically induced pluripotent stem cells using small molecule inhibitors and activators that target either downstream protein kinases or modify chromatin structure to promote somatic cell reprogramming. In addition, epigenetic modulating small molecule compounds that enhance cellular reprogramming and functionally replace some reprogramming factors are discussed.
Greenberg, Brittany E. , "Reprogramming to Pluripotency Using Small Molecule Compounds" (2015). Theses and Dissertations (ETD). Paper 97. http://dx.doi.org/10.21007/etd.cghs.2015.0117.