Date of Award

6-2019

Document Type

Thesis

Degree Name

Master of Science (MS)

Program

Biomedical Sciences

Track

Cancer and Developmental Biology

Research Advisor

Taosheng Chen, Ph.D.

Committee

Meiyun Fan, Mark Hatley

Keywords

CYP3A5, PDAC

Abstract

CYP3A5 is a drug metabolism enzyme that is polymorphically expressed in normal tissues and expressed in high amounts in some cancers, including pancreatic ductal adenocarcinoma (PDAC). There have been several published studies regarding CYP3A5 regulators, but there is still much to be discovered concerning CYP3A5 regulation in cancer. To further understand the regulation of CYP3A5, a high-throughput screen was performed in the AsPC-1 PDAC cell line. This screen employed small interfering RNA (siRNA) targeting transcription factors and chromatin-associated proteins. We identified siRNA that decreased CYP3A5 mRNA and protein levels in AsPC-1 cells and probed into the potential mechanisms. Taken together, this study brought to light a previously undescribed regulator of CYP3A5 expression.

Declaration of Authorship

Declaration of Authorship is included in the supplemental files.

ORCID

0000-0001-6884-7310

DOI

10.21007/etd.cghs.2019.0475

Additional Files
2019-012-Hoyer-DOA.pdf (511 kB)
Declaration of Authorship
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