Date of Award
Master of Science (MS)
Cancer and Developmental Biology
Taosheng Chen, Ph.D.
Meiyun Fan, Mark Hatley
CYP3A5 is a drug metabolism enzyme that is polymorphically expressed in normal tissues and expressed in high amounts in some cancers, including pancreatic ductal adenocarcinoma (PDAC). There have been several published studies regarding CYP3A5 regulators, but there is still much to be discovered concerning CYP3A5 regulation in cancer. To further understand the regulation of CYP3A5, a high-throughput screen was performed in the AsPC-1 PDAC cell line. This screen employed small interfering RNA (siRNA) targeting transcription factors and chromatin-associated proteins. We identified siRNA that decreased CYP3A5 mRNA and protein levels in AsPC-1 cells and probed into the potential mechanisms. Taken together, this study brought to light a previously undescribed regulator of CYP3A5 expression.
Hoyer, Jessica D. (0000-0001-6884-7310), "Regulation of CYP3A5 in Pancreatic Ductal Adenocarcinoma" (2019). Theses and Dissertations (ETD). Paper 483. http://dx.doi.org/10.21007/etd.cghs.2019.0475.