In vitro Incorporation of Iron-59 into Hemoglobin S Visualized by Autoradiography
UTHSC Affiliation
College of Medicine
Document Type
Article
Publication Date
9-8-1955
Publication Title
Proceedings of the Society for Experimental Biology and Medicine
Volume
89
Issue
4
Abstract
1. Human bone marrow of a patient with sickle cell anemia and peripheral blood of a normal individual were incubated with iron-59 under similar conditions. Auto-radiograms were prepared from the cells. After incubation, hemolysates of the cells were fractionated by paper electrophoresis and the radioactivity of the fractions was measured. 2. 90% of the total radioactivity was located in hemoglobin from bone marrow hemoly sates. At 37°C the rate of hemoglobin synthesis as measured by iron-5 9 utilization is more rapid in the most immature erythrocytic cells beginning with the rubriblast. The rate of synthesis at 0°C was 25% of that at 37°C. 3. Less than 5% of the radioactive iron in incubated normal peripheral blood was in the hemoglobin fraction. © 1955, SAGE Publications. All rights reserved.
Recommended Citation
Kraus, L.,
&
Morrison, D.
(1955).
In vitro Incorporation of Iron-59 into Hemoglobin S Visualized by Autoradiography.
Proceedings of the Society for Experimental Biology and Medicine,
89(4).
http://doi.org/10.3181/00379727-89-21887
Retrieved from: https://dc.uthsc.edu/fac_pubs/299