ORCID
http://orcid.org/0000-0002-6368-3043
Publication Date
Spring 3-16-2018
Project Category
Health Research
Faculty Mentor
Deidra Mountain, PhD
Document Type
Poster
Abstract
Introduction:
My project was a continuation of the Vascular Research Lab’s (VRL) ongoing research at the University of Tennessee Medical Center Knoxville (UTMCK) aimed at optimizing liposomal encapsulation efficiency of small interfering RNA (siRNA) which can be used to silence genes to prevent a variety of disease pathologies.
Methods:
- Assay siRNA loading capacity of liposomes based on lipid concentration
- Development of a method for liposome purification: HPLC & HiTRAP Column
Results & Conclusion:
- siRNA loading capacity
- Higher lipid:siRNA resulted in increased encapsulation efficiency
- HPLC – did not work as expected
- HiTRAP Column – currently being optimized to be used as part of standard operating procedures
Recommended Citation
Joyce, Christine Sharrett and Mountain, Deidra PhD (http://orcid.org/0000-0002-6368-3043), "Optimization of Liposomal Encapsulation Efficiency" (2018). Longitudinal Scholar's Project. Paper 3. http://dx.doi.org/10.21007/com.lsp.2018.0002.
https://dc.uthsc.edu/lsp/3