Studies on Acid Hydrolysates of Vesicant-treated Insulin
UTHSC Affiliation
College of Medicine
Document Type
Article
Publication Date
1-1-1948
Publication Title
Obstetrical and Gynecological Survey
Volume
3
Issue
2
Abstract
Insulin which had been treated with a minute amount of n-butyl ß-chloroethyl sulfide (butyl-H) containing radiosulfur was subjected to acid hydrolysis. By application of the “washing-out” technique, evidence was obtained for the presence of radiosulfur-containing N-(ß-butylmercapto)- ethyl-phenylalanine in the hydrolysate. Various control experiments provided no evidence for the attachment of vesicant residues to the amino group of phenylalanine during the hydrolysis procedure. Therefore it was concluded that in the vesicant-treated insulin, a fraction of the vesicant had been attached to the free amino group of some of the phenylalanine moieties in the intact protein molecule. © 1948, American Chemical Society. All rights reserved.
Recommended Citation
Stevens, C.,
Wood, J.,
Rachele, J.,
&
du Vigneaud, V.
(1948).
Studies on Acid Hydrolysates of Vesicant-treated Insulin.
Obstetrical and Gynecological Survey,
3(2).
http://doi.org/10.1097/00006254-194804000-00032
Retrieved from: https://dc.uthsc.edu/fac_pubs/166