Studies on Acid Hydrolysates of Vesicant-treated Insulin
Document Type
Article
Publication Date
7-1-1948
Publication Title
Journal of the American Chemical Society
Volume
70
Issue
7
Abstract
Insulin which had been treated with a minute amount of n-butyl ß-chloroethyl sulfide (butyl-H) containing radiosulfur was subjected to acid hydrolysis. By application of the “washing-out” technique, evidence was obtained for the presence of radiosulfur-containing N-(ß-butylmercapto)- ethyl-phenylalanine in the hydrolysate. Various control experiments provided no evidence for the attachment of vesicant residues to the amino group of phenylalanine during the hydrolysis procedure. Therefore it was concluded that in the vesicant-treated insulin, a fraction of the vesicant had been attached to the free amino group of some of the phenylalanine moieties in the intact protein molecule. © 1948, American Chemical Society. All rights reserved.
Recommended Citation
Stevens, C.,
Wood, J.,
Rachele, J.,
&
Vincent du, V.
(1948).
Studies on Acid Hydrolysates of Vesicant-treated Insulin.
Journal of the American Chemical Society,
70(7).
http://doi.org/10.1021/ja01187a076
Retrieved from: https://dc.uthsc.edu/fac_pubs/166